Elevated resting and agonist-induced whole blood chemiluminescence in patients with active infective endocarditis
Stanislaw Ostrowski 1,
Marek Kasielski 1,
Jacek Kordiak 1,
Dariusz Nowak 1*
1 Medical University of Lodz, Poland
* To whom correspondence should be addressed. E-mail: dnowak{at}zdn.am.lodz.pl.
 |
Abstract |
|---|
Infective endocarditis (IE) and surgical procedures related to cardiac surgery are accompanied by inflammatory responses that may alter production of oxidants by phagocytes. This study evaluates luminol enhanced whole blood chemiluminescence (LBCL) as a measure of oxidative production by circulating phagocytes in 26 IE patients in comparison to 27 matched patients with acquired valvular heart disease and 25 healthy controls. Blood was collected the day before and 3, 7, 12, and 21 days after valve replacement surgery for LBCL measurement; resting (rCL) and agonist (fMLP)-stimulated total light emission (tCL). Preoperative rCL and tCL with values observed after 3, 7, 14, and 21 days from surgery were higher (p<0.01) in patients with IE than in healthy controls. Median preoperative rCL, and tCL was about 2.5-times higher (p<0.01) in IE group than in patients with valvular heart disease (4.3 vs. 1.7 units/104 phagocytes and 2473 vs. 782 units x s/104 phagocytes). Three days after valve replacement, LBCL rose 3 times (p<0.01) in both operated groups. With patient recovery, LBCL decreased and no differences were noted between groups. Patients with IE had elevated LBCL reflecting increased oxidants release from circulating phagocytes that may predispose to the development of oxidative stress. Key words: Infective endocarditis; Whole blood chemiluminescence; Valvular heart disease; Valve replacement; Oxidative stress; Circulating phagocytes
