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Expression of endothelial cell-specific adhesion molecules in lungs after cardiac arrest

^a Department of Organ Preservation Technology, Graduate School of Medicine, Kyoto University, 54 Shogoin-Kawahara-cho, Kyoto 606-8507, Japan
^b Department of Thoracic Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan

*Corresponding author. Tel.: +81-75-751-3358; fax: +81-75-751-4647.

E-mail address: bando{at}kuhp.kyoto-u.ac.jp (T. Bando).

Objectives: A method to compensate for donor shortages could be donation after cardiac death. In this study, we considered endothelial cell-specific molecules, claudin-5 and VE-cadherin, as possible biomarkers predicting lung injury against warm ischemia. We investigated how the expression of these molecules could change after cardiac arrest in a mouse lung, comparing other molecules presumably relating with ischemia. Methods: At given intervals after cardiac arrest, the lungs were harvested. Quantitative analysis of mRNA expression of claudin-5, VE-cadherin, IL-1β, IL-10, HIF-, Egr-1, VEGF, Ang-1 and Ang-2 genes in lung tissues with several periods of warm ischemia was performed. Results: Regarding endothelial cell-specific molecules, there were significant differences in both claudin-5 and VE-cadherin mRNA expression between 0 h and 4 h after cardiac arrest. IL-1β mRNA expression 1 h, 2 h and 4 h after cardiac arrest increased significantly, compared with that at 0 h. There were no significant differences with the other genes. Conclusions: We found that it took more time for claudin-5 and VE-cadherin mRNA expression to change significantly than IL-1β mRNA expression; therefore, endothelial cell-specific molecules, claudin-5 and VE-cadherin, might be no better candidates for clinical use than IL-1β.

Key Words: Lung preservation; Ischemia; Lung; Non-heart-beating donor; Endothelium; Donation after cardiac death