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Sustained-release form of basic fibroblast growth factor prevents catheter-related bacterial invasion in mice

^a Department of Cardiovascular Surgery, Kyoto University Graduate School of Medicine, 54 Shogoin-Kawara-cho, Sakyo-ku Kyoto, 606-8507, Japan
^b Department of Laboratory Science, Kyoto University Graduate School of Medicine, Kyoto, Japan
^c Department of Microbiology, Kyoto University Graduate School of Medicine, Kyoto, Japan
^d The Institute for Frontier Medical Science, Kyoto University, Kyoto, Japan

*Corresponding author. Tel.: +81-75-751-3780; fax: +81-75-751-3098.

E-mail address: komelab{at}kuhp.kyoto-u.ac.jp (M. Komeda).

Catheter-related infection is a frequent and serious complication. One factor responsible for catheter-related infection is bacterial invasion at the catheter-insertion site. We have shown that the sustained-release form of basic fibroblast growth factor (bFGF) enhances tissue regeneration and angiogenesis in various pathological conditions. The purpose of this study was to determine whether topical use of sustained-release form of bFGF promotes tissue regeneration around the wound and prevents catheter-related bacterial invasion. Fifty-four male mice (C57BL/6) were divided into three groups according to what was implanted subcutaneously on the back (each group, n=18): a Dacron sheet alone (group A), a Dacron sheet and a plain gelatin sheet (group B), and a Dacron sheet and sustained-release of bFGF (50 µg) (group C). Seven days after the implantation, the tissue immediately above the Dacron sheet was inoculated with methicillin-resistant Staphylococcus aureus (MRSA). In histological examinations, group C had a larger granulation tissue area containing a larger amount of collagen tissue and vessels than the other groups. Two days after the MRSA inoculation, the number of MRSA in the Dacron sheet of group C was significantly smaller than the other groups (P<0.01). Pretreatment with sustained-release form of bFGF may prevent catheter-related bacterial invasion.

Key Words: Tissue regeneration; Growth factor; Sustained-release form of bFGF; Catheter-related infection