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Gene transfer of ß₂ adrenergic receptor enhances cardioprotective effects of ischemic preconditioning in rat hearts after myocardial infarction

^a Department of Thoracic and Cardiovascular Surgery, and Chemistry, Osaka Medical College, 2-7 Daigakucho, Takatsuki, Osaka 569-8686, Japan
^b Department of Surgery, Course of Interventional Medicine (E1), Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan

*Corresponding author. Tel.: +81-726-83-1221; fax: +81-726-84-6542.

E-mail address: tho050{at}poh.osaka-med.ac.jp (S. Mieno).

Objective: The purpose of the present study was to determine a role of ß₂ adrenergic receptor (ß₂AR) in ischemic preconditioning (IPC) response. Methods: Post-myocardial infarction (MI) hearts were produced by ligating the left anterior descending coronary artery for 2 weeks. Post-MI hearts were transfected with the empty virus (Empty-vivo) or ß₂AR cDNA (ß₂AR-vivo) by intracoronary infusion of hemagglutinating virus of Japan-liposome. Empty-vivo or ß₂AR-vivo hearts were subjected to Langendorff perfusion as Control or ß₂AR hearts, respectively. IPC was undertaken in Control(IPC) and ß₂AR(IPC+ß₂AR). After global ischemia, seven hearts in each group were reperfused and normalized left ventricular peak developed pressures (LVPDP) and creatine phosphokinase (CPK) leakage were measured. ß₂AR gene transfection was confirmed by measuring responsiveness to isoproterenol, real time RT-PCR and immunohistochemistory. Results: IPC preserved LVPDP and reduced CPK leakage in IPC+ß₂AR hearts as compared with IPC hearts. LVPDP was decreased in addition to increase in CPK leakage in ß₂AR hearts as compared with Control. Expression of ß₂AR and responsiveness to isoproterenol were increased in ß₂AR-vivo as compared with Empty-vivo hearts. Conclusion: These results indicate that ß₂AR are required to generate IPC effects, and that ß₂AR gene transfection enhances IPC effects in post-MI hearts.

Key Words: ß₂ adrenergic receptor; Gene transfection; Ischemia; Ischemic preconditioning; Myocardial infarction

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