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Interact CardioVasc Thorac Surg 2005;4:163-167. doi:10.1510/icvts.2004.096792
© 2005 European Association of Cardio-Thoracic Surgery
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Yoshiki Sawa
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Right arrow Myocardial protection

Work in progress report - Experimental

Gene transfer of ß2 adrenergic receptor enhances cardioprotective effects of ischemic preconditioning in rat hearts after myocardial infarction

Shigetoshi Mienoa,*, Fusao Watanabea, Yoshiki Sawab and Hitoshi Horimotoa

a Department of Thoracic and Cardiovascular Surgery, and Chemistry, Osaka Medical College, 2-7 Daigakucho, Takatsuki, Osaka 569-8686, Japan
b Department of Surgery, Course of Interventional Medicine (E1), Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan

*Corresponding author. Tel.: +81-726-83-1221; fax: +81-726-84-6542.

E-mail address: tho050{at}poh.osaka-med.ac.jp (S. Mieno).

Objective: The purpose of the present study was to determine a role of ß2 adrenergic receptor (ß2AR) in ischemic preconditioning (IPC) response. Methods: Post-myocardial infarction (MI) hearts were produced by ligating the left anterior descending coronary artery for 2 weeks. Post-MI hearts were transfected with the empty virus (Empty-vivo) or ß2AR cDNA (ß2AR-vivo) by intracoronary infusion of hemagglutinating virus of Japan-liposome. Empty-vivo or ß2AR-vivo hearts were subjected to Langendorff perfusion as Control or ß2AR hearts, respectively. IPC was undertaken in Control(IPC) and ß2AR(IPC+ß2AR). After global ischemia, seven hearts in each group were reperfused and normalized left ventricular peak developed pressures (LVPDP) and creatine phosphokinase (CPK) leakage were measured. ß2AR gene transfection was confirmed by measuring responsiveness to isoproterenol, real time RT-PCR and immunohistochemistory. Results: IPC preserved LVPDP and reduced CPK leakage in IPC+ß2AR hearts as compared with IPC hearts. LVPDP was decreased in addition to increase in CPK leakage in ß2AR hearts as compared with Control. Expression of ß2AR and responsiveness to isoproterenol were increased in ß2AR-vivo as compared with Empty-vivo hearts. Conclusion: These results indicate that ß2AR are required to generate IPC effects, and that ß2AR gene transfection enhances IPC effects in post-MI hearts.

Key Words: ß2 adrenergic receptor; Gene transfection; Ischemia; Ischemic preconditioning; Myocardial infarction

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