Interactive Cardiovascular and Thoracic Surgery 3:612-615(2004)
© 2004 European Association of Cardio-Thoracic Surgery
Work in progress report - Cardiac general |
A novel method of ‘preparative’ myocardial protection using green tea polyphenol in oral uptake
Senri Miwa,
Kazuhiro Yamazaki,
Suong-Hyu Hyon and
Masashi Komeda*
Department of Cardiovascular Surgery, Graduate School of Medicine, Kyoto University, 54 Kawaharacho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan
* Corresponding author. Tel.: +81-75-751-3780; fax: +81-75-751-3098. (E-mail: masakom{at}kuhp.kyoto-u.ac.jp).
Reperfusion injury has been one of the serious problems in cardiac surgery and medicine. We report a novel method of myocardial protection. Eight Sprague–Dawley rats, group A, were administrated green tea polyphenol orally. Eight other rats, group B, received no medication. Isolated hearts were perfused with a Langendorff's apparatus, and reperfused after 90min arrest. The left ventricular (LV) function, size, weight and the oxidative stress spaciotemporal analysis were performed. The LV size in group B became enlarged by 1.8±0.12 (mean±SD), but in group A, the LV enlarged only 1.1±0.08 times. The heart weight ratio was lighter (1.35±0.05) in group A than in group B (1.49±0.03, P<0.05). The LV end-systolic pressure volume relationship remained higher in group A. Oxidative stress as shown by 8-hydroxy-2'-deoxyguanosine was lower in group A (81.5±11.6) than in group B (226.9±35.6, P<0.0001). Polyphenol labeled FITC was located in the cell membrane of cardiomyocite. Polyphenol can protect a heart from oxidative stress, and maintain good LV function after ischemic arrest and reperfusion. This pre-treatment by polyphenol may add further benefit to current treatments of myocardial protection.
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