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Clinical characteristics relevant to myocardial cell apoptosis: analysis of pericardial fluid

^a Department of Cardiovascular Surgery, Kyoto University, Kyoto, Japan
^b Department of Cardiovascular Medicine, Kyoto University, Kyoto, Japan
^c School of Health Sciences, Faculty of Medicine, Kyoto University, 53 Kawaharacho Shogoin, Sakyo-ku, Kyoto 606-8507, Japan
^d Division of Cardiovascular Surgery, Takeda Hospital, Kyoto, Japan

^* Corresponding author. Tel.: +81-75-751-3932; fax: +81-75-751-3909
mfujita{at}kuhp.kyoto-u.ac.jp

We investigated the pro-apoptotic potential of pericardial fluids from patients with different clinical conditions on cultured neonatal rat cardiomyocytes. Pericardial fluids were obtained during open heart surgery from 88 patients with ischemic heart disease valvular heart disease or aortic disease The terminal deoxynucleotidyl transfer-mediated end labeling fragmented nuclei assay was performed on primary cardiac myocytes from neonatal rats in the presence of 1% pericardial fluid from each patient. We evaluated relations between these patients' clinical characteristics and the extent of myocardial cell apoptosis. Induction of myocardial cell apoptosis by pericardial fluids was observed in 29 of the 88 patients (33.0%). The prevalence of myocardial cell apoptosis was significantly influenced by diabetes mellitus (DM) (53.6% with vs. 23.3% without, ), acute coronary syndrome (ACS) (64.7% with vs. 25.4% without, ), and poor left ventricular systolic function (60.0% with vs. 25.0% without, ). Multivariate stepwise logistic regression analysis revealed that the presence of DM, ACS, and poor left systolic function were significant predictors of myocardial cell apoptosis. DM, ACS and left ventricular dysfunction may play important roles in the pathogenesis of myocardial cell apoptosis in the clinical setting.

Key Words: Angina; Apoptosis; Diabetes mellitus; Left ventricular function; Pericardium